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Structure and alternative splicing of the presenilin-2 gene.
Missense mutations in the presenilin-1 (PS-1) and presenilin-2 (PS-2) genes have been shown to be causes of autosomal dominant Alzheimer's disease (the AD3 and AD4 loci, respectively). Alternative splicing has previously been reported in the PS-1 gene. In this study, elucidation of intron/exon boundary sequences revealed that PS-2 is encoded by 10 coding exons. In addition, PS-2 cDNA cloning and RT-PCR using RNA from a variety of normal tissues revealed the presence of alternatively spliced...
Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library.
A human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs) was evaluated by partial sequencing of over 1,600 clones. Advantages of this library, constructed for EST sequencing, include the use of directional cloning, size selection, very low numbers of mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants and a broad representation of transcripts. 37% of the clones were identified, based on matches to over 320 different...
Hamilton O. Smith Fund
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About Jeffrey M. Hoffman
Jeff Hoffman did his undergraduate and graduate work at Louisiana State University in microbiology. He started work in April 2002 and was the first person hired at the Venter Institute. Since 2002 Jeff has been the Expedition Scientist onboard Sorcerer II. His time on Sorcerer II includes the pilot study in the Sargasso Sea, the circumnavigation around the world from 2004-2006 and the Baltic/Mediterranean/ Black Sea of 2009-2010.
Meeting of the Presidential Commission for the Study of Bioethical Issues
Date: Thursday, July 8, 2010 Friday, July 9, 2010 Time: 8:30 am — 5:15 pm (Thursday, July 8) 9 am — 12:15 pm (Friday, July 9) Location: The Ritz-Carlton Washington, DC 1150 22nd Street, NW Washington, DC 20037 202-835-0500 Topic: Synthetic Biology The Presidential Commission for the Study of Bioethical Issues will hold its first meeting on July 8-9 in Washington, DC. The primary topic is synthetic biology. Speakers include Craig Venter, PhD,...
Stalking the fourth domain in metagenomic data: searching for, discovering, and interpreting novel, deep branches in marker gene phylogenetic trees.
Most of our knowledge about the ancient evolutionary history of organisms has been derived from data associated with specific known organisms (i.e., organisms that we can study directly such as plants, metazoans, and culturable microbes). Recently, however, a new source of data for such studies has arrived: DNA sequence data generated directly from environmental samples. Such metagenomic data has enormous potential in a variety of areas including, as we argue here, in studies of very early...
Aggressive assembly of pyrosequencing reads with mates.
DNA sequence reads from Sanger and pyrosequencing platforms differ in cost, accuracy, typical coverage, average read length and the variety of available paired-end protocols. Both read types can complement one another in a 'hybrid' approach to whole-genome shotgun sequencing projects, but assembly software must be modified to accommodate their different characteristics. This is true even of pyrosequencing mated and unmated read combinations. Without special modifications, assemblers tuned...
JCVI Promotes Science Literacy in the U.S.
The issue of our society’s science literacy continues to circulate through the media. Recently, reporters focused on results of the Pew Research Center’s Science Knowledge Quiz, which indicates that most Americans would score a grade of C on a basic science test. The gender and racial gaps revealed by the study were equally discouraging. Our planet is in crisis, and we need to mobilize all our intellectual forces to save it. One solution could lie in building a scientifically...