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Biowalk of Fame
There is a new “Biowalk of Fame” in Maryland, and our own Craig Venter was one of the first honorees receiving a plaque, which is there for all to see as you stroll through lovely Silver Spring. Other honorees include Dr. Martin Rodbell and Ben Carson. The event to honor the awardees was on April 22, which also it happens to be Earth Day. Although it rained heavily throughout the event, there were a large number of people in attendance including several local government...
Scientists develop most complete whole-cell computer simulation model of cell to date
LA JOLLA, CA—January 20, 2022—Scientists from the University of Illinois at Urbana-Champaign, J. Craig Venter Institute, and Technische Universität Dresden have developed a computer model of the minimized synthetic cell, JCVI-syn3A, that accurately predicts the growth and molecular structure of its real-life analog. It is the most complete computer-simulated whole-cell model to date. The findings have been published in the journal Cell. The JCVI team has been doggedly pursuing...
JCVI Scientists Publish First Bacterial Genome Transplantation Changing One Species to Another
ROCKVILLE, MD — June 28, 2007 — Researchers at the J. Craig Venter Institute (JCVI) today announced the results of work on genome transplantation methods allowing them to transform one type of bacteria into another type dictated by the transplanted chromosome. The work, published online in the journal Science, by JCVI's Carole Lartigue, Ph.D. and colleagues, outlines the methods and techniques used to change one bacterial species, Mycoplasma capricolum into another, Mycoplasma...
Of Jaws and Man
SINGAPORE AND ROCKVILLE, MD, December 21, 2006 — A joint team of scientists from Singapore A*STAR's Institute of Molecular and Cell Biology (IMCB) and the United States-based J. Craig Venter Institute have completed an initial sequence of the elephant shark genome. By comparing this genome to the human genome they have discovered a large number of ancient DNA fragments in the human genome. These ancient DNA fragments in the human genome do not make proteins; instead they regulate...
Multiple personal genomes await.
Publisher Correction: Voices of biotech leaders.
Enzymatic assembly of DNA molecules up to several hundred kilobases.
We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.
Shotgun sequencing of the human genome.
Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.
Direct cell-to-cell transfer of genomes from bacteria to yeast facilitates genome engineering for bacteria that are not amenable to genetic manipulation by allowing instead for the utilization of the powerful yeast genetic tools. Here we describe a protocol for transferring whole genomes from bacterial cells to yeast spheroplasts without any DNA purification process. The method is dependent on the treatment of the bacterial and yeast cellular mixture with PEG, which induces cell fusion,...