Publications

Methods in molecular biology (Clifton, N.J.). 2014-01-01; 1140.75-88.

Expression and solubility testing in a high-throughput environment

Kwon K, Peterson SN

PMID: 24590710

Abstract

The expression and screening of the solubility of recombinant proteins is an important step in the high-throughput (HT) production of target proteins. For many applications, E. coli remains the most widely used expression system due to the relative ease of adapting it to HT pipelines. Herein is described a platform using a 96-well format for efficient expression and solubility screening of target proteins.

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