PAST PROJECT

Burkholderia Genome Sequencing and Analysis

We developed some of the earliest annotated genome sequences for the genus Burkholderia including the biothreat agents Burkholderia mallei and Burkholderia pseudomallei. We expanded on our genome sequencing efforts to include efforts in whole genome gene expression analysis and functional analysis of genome variation. We also sequenced several members of the Burkholderia cenocepacia complex that are important pathogens in the lungs of cystic fibrosis patients.

Publications

Frontiers in microbiology. 2015-01-01; 6.1338.

The In vitro Antibiotic Tolerant Persister Population in Burkholderia pseudomallei is Altered by Environmental Factors

Nierman WC, Yu Y, Losada L

PMID: 26696964

PLoS genetics. 2014-09-01; 10.9: e1004640.

The tandem repeats enabling reversible switching between the two phases of β-lactamase substrate spectrum

Yi H, Song H, Hwang J, Kim K, Nierman WC, Kim HS

PMID: 25233343

Genome announcements. 2013-11-07; 1.6:

Sequence Determination of Burkholderia pseudomallei Strain NCTC 13392 Colony Morphology Variants

Vipond J, Kane J, Hatch G, McCorrison J, Nierman WC, Losada L

PMID: 24201202

Genome announcements. 2013-10-17; 1.5:

Draft Genome Sequences of Burkholderia cenocepacia ET12 Lineage Strains K56-2 and BC7

Varga JJ, Losada L, Zelazny AM, Kim M, McCorrison J, Brinkac L, Sampaio EP, Greenberg DE, Singh I, Heiner C, Ashby M, Nierman WC, Holland SM, Goldberg JB

PMID: 24136849

Journal of bacteriology. 2012-11-01; 194.22: 6356-7.

Draft genome sequence determination for cystic fibrosis and chronic granulomatous disease Burkholderia multivorans isolates

Varga JJ, Losada L, Zelazny AM, Brinkac L, Harkins D, Radune D, Hostetler J, Sampaio EP, Ronning CM, Nierman WC, Greenberg DE, Holland SM, Goldberg JB

PMID: 23105085

PloS one. 2012-05-22; 7.5: e37585.

Twelve positions in a β-lactamase that can expand its substrate spectrum with a single amino acid substitution

Yi H, Cho KH, Cho YS, Kim K, Nierman WC, Kim HS

PMID: 22629423

Molecules and cells. 2009-02-28; 27.2: 237-41.

Simple sequence repeat (SSR)-based gene diversity in Burkholderia pseudomallei and Burkholderia mallei

Song H, Hwang J, Myung J, Seo H, Yi H, Sim HS, Kim BS, Nierman WC, Kim HS

PMID: 19277507

BMC genomics. 2008-11-27; 9.566.

Genomic islands from five strains of Burkholderia pseudomallei

Tuanyok A, Leadem BR, Auerbach RK, Beckstrom-Sternberg SM, Beckstrom-Sternberg JS, Mayo M, Wuthiekanun V, Brettin TS, Nierman WC, Peacock SJ, Currie BJ, Wagner DM, Keim P

PMID: 19038032

PloS one. 2008-04-30; 3.4: e2058.

Characterization of clinically-attenuated Burkholderia mallei by whole genome sequencing: candidate strain for exclusion from Select Agent lists

Schutzer SE, Schlater LR, Ronning CM, DeShazer D, Luft BJ, Dunn JJ, Ravel J, Fraser-Liggett CM, Nierman WC

PMID: 18446194

Funding

Funding for this project provided through the National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID).