| Accession | TIGR03283 |
| Name | thy_syn_methano |
| Function | thymidylate synthase, methanogen type |
| Trusted Cutoff | 174.55 |
| Domain Trusted Cutoff | 174.55 |
| Noise Cutoff | 112.65 |
| Domain Noise Cutoff | 112.65 |
| Isology Type | equivalog |
| EC Number | 2.1.1.- |
| HMM Length | 201 |
| Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
| Subrole Category | 2'-Deoxyribonucleotide metabolism |
| Gene Ontology Term | GO:0004799: thymidylate synthase activity molecular_function |
| | GO:0009265: 2'-deoxyribonucleotide biosynthetic process biological_process |
| Author | Haft DH |
| Entry Date | Jan 22 2007 12:08PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Thymidylate synthase makes dTMP for DNA synthesis, and is among the most widely distributed of all enzymes. Members of this protein family are encoded within a completed genome sequence if and only if that species is one of the methanogenenic archaea. In these species, tetrahydromethanopterin replaces tetrahydrofolate, The member from Methanobacterium thermoautotrophicum was shown to behave as a thymidylate synthase based on similar side reactions (the exchange of a characteristic proton with water), although the full reaction was not reconstituted. Partial sequence data showed no similarity to known thymidylate synthases simply because the region sequenced was from a distinctive N-terminal region not found in other thymidylate synthases. Members of this protein family appear, therefore, to a novel, tetrahydromethanopterin-dependent thymidylate synthase. |
| References | RN [1]
RM PMID: 8143733
RT Purification and partial characterization of a putative thymidylate synthase from Methanobacterium thermoautotrophicum.
RA Krone UE, McFarlan SC, Hogenkamp HP
RL Eur J Biochem. 1994 Mar 15;220(3):789-94.
DR HAMAP; MF_00008; 8 of 351 |
