| Accession | TIGR02884 |
| Name | spore_pdaA |
| Function | delta-lactam-biosynthetic de-N-acetylase |
| Gene Symbol | pdaA |
| Trusted Cutoff | 298.00 |
| Domain Trusted Cutoff | 298.00 |
| Noise Cutoff | 164.95 |
| Domain Noise Cutoff | 164.95 |
| Isology Type | equivalog |
| EC Number | 3.5.-.- |
| HMM Length | 224 |
| Mainrole Category | Cell envelope |
| Subrole Category | Biosynthesis and degradation of murein sacculus and peptidoglycan |
| Author | Haft DH |
| Entry Date | Mar 10 2006 9:54AM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Muramic delta-lactam is an unusual constituent of peptidoglycan, found only in bacterial spores in the peptidoglycan wall, or spore cortex. The proteins in this family are PdaA (yfjS), a member of a larger family of polysaccharide deacetylases, and are specificially involved in delta-lactam biosynthesis. PdaA acts immediately after CwlD, an N-acetylmuramoyl-L-alanine amidase and performs a de-N-acetylation. PdaA may also perform the following transpeptidation for lactam ring formation, as heterologous expression in E. coli of CwlD and PdaA together is sufficient for delta-lactam production. |
| References | DR PFAM; PF01522; Polysacc_deac_1;
RN [1]
RM 14679227
RT Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
RA Gilmore ME, Bandyopadhyay D, Dean AM, Linnstaedt SD, Popham DL.
RL J Bacteriol. 2004 Jan;186(1):80-9.
RN [2]
RM 12374835
RT A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
RA Fukushima T, Yamamoto H, Atrih A, Foster SJ, Sekiguchi J.
RL J Bacteriol. 2002 Nov;184(21):6007-15. |
