Accession | TIGR01718 |
Name | Uridine-psphlse |
Function | uridine phosphorylase |
Gene Symbol | udp |
Trusted Cutoff | 280.30 |
Domain Trusted Cutoff | 280.30 |
Noise Cutoff | 209.40 |
Domain Noise Cutoff | 209.40 |
Isology Type | equivalog |
EC Number | 2.4.2.3 |
HMM Length | 245 |
Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
Subrole Category | Salvage of nucleosides and nucleotides |
Gene Ontology Term | GO:0004731: purine-nucleoside phosphorylase activity molecular_function |
| GO:0006139: nucleobase-containing compound metabolic process biological_process |
Author | Selengut J |
Entry Date | Oct 4 2002 4:53PM |
Last Modified | Feb 14 2011 3:27PM |
Comment | This model represents a family of bacterial and archaeal uridine phosphorylases unrelated to the mammalian enzymes of the same name. The E. coli [1], Salmonella [2] and Klebsiella [3] genes have been characterized.
Sequences from Clostridium, Streptomyces, Treponema, Halobacterium and Pyrobaculum were included above trusted on the basis of sequence homology and a PAM-based neighbor-joining tree. A clade including second sequences from Halobacterium and Vibrio was somewhat more distantly related and may represent a slightly different substrate specificity - these were placed below the noise cutoff. More distantly related is a clade of archaeal sequences which as related to the DeoD family of inosine phosphorylases (TIGR00107) as they are to these uridine phosphorylases. This clade includes a characterized protein from Sulfolobus solfataricus which has been mis-named as a methylthioadenosine phosphorylase, but which acts on inosine and guanosine - it is unclear whether uridine has been evaluated as a substrate [4]. |
References | RN [1]
RM PMID: 16751
RT Uridine phosphorylase from Escherichia coli. Physical and chemical characterization.
RA Leer JC, Hammer-Jespersen K, Schwartz M.
RL Eur J Biochem 1977 May 2;75(1):217-24
RN [2]
RM PMID: 9526114
RT Isolation and initial characterization of the uridine phosphorylase from Salmonella typhimurium.
RA Molchan OK, Dmitrieva NA, Romanova DV, Lopes LE, Debabov VG, Mironov AS.
RL Biochemistry (Mosc) 1998 Feb;63(2):195-9
RN [3]
RM PMID: 8534998
RT Molecular cloning and nucleotide sequence of purine nucleoside phosphorylase and uridine phosphorylase genes from Klebsiella sp.
RA Takehara M, Ling F, Izawa S, Inoue Y, Kimura A.
RL Biosci Biotechnol Biochem. 1995 Oct;59(10):1987-90.
RN [4]
RM PMID: 7929153
RT Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds.
RA Cacciapuoti G, Porcelli M, Bertoldo C, De Rosa M, Zappia V.
RL J Biol Chem. 1994 Oct 7;269(40):24762-9. |