| Accession | TIGR00432 |
| Name | arcsn_tRNA_tgt |
| Function | tRNA-guanine(15) transglycosylase |
| Gene Symbol | tgtA |
| Trusted Cutoff | 350.00 |
| Domain Trusted Cutoff | 350.00 |
| Noise Cutoff | 200.00 |
| Domain Noise Cutoff | 200.00 |
| Isology Type | equivalog |
| EC Number | 2.4.2.48 |
| HMM Length | 637 |
| Mainrole Category | Protein synthesis |
| Subrole Category | tRNA and rRNA base modification |
| Gene Ontology Term | GO:0006400: tRNA modification biological_process |
| | GO:0016763: transferase activity, transferring pentosyl groups molecular_function |
| Author | Haft DH |
| Entry Date | Apr 20 1999 2:06PM |
| Last Modified | Dec 21 2011 12:30PM |
| Comment | This tRNA-guanine transglycosylase (tgt) differs from the tgt of E. coli and other Bacteria in the site of action and the modification that results. It exchanges 7-cyano-7-deazaguanine (preQ0) with guanine at position 15 of archaeal tRNA; this nucleotide is subsequently converted to archaeosine, found exclusively in the Archaea. This enzyme from Haloferax volcanii has been purified, characterized, and partially sequenced and is the basis for identifying this family. In contrast, bacterial tgt (TIGR00430) catalyzes the exchange of preQ0 or preQ1 for the guanine base at position 34; this nucleotide is subsequently modified to queuosine. Archeoglobus fulgidus has both enzymes, while some other Archaea have just this one. |
| References | RM Medline; 97390456
SE TIGR
GA hmmls
AL clustalw
DR HAMAP; MF_01634; 19 of 32 |
