Accession | TIGR00342 |
Name | TIGR00342 |
Function | tRNA sulfurtransferase ThiI |
Gene Symbol | thiI |
Trusted Cutoff | 264.50 |
Domain Trusted Cutoff | 264.50 |
Noise Cutoff | 166.10 |
Domain Noise Cutoff | 166.10 |
Isology Type | equivalog_domain |
EC Number | 2.8.1.4 |
HMM Length | 373 |
Mainrole Category | Biosynthesis of cofactors, prosthetic groups, and carriers |
Subrole Category | Thiamine |
Gene Ontology Term | GO:0003824: catalytic activity molecular_function |
| GO:0006400: tRNA modification biological_process |
| GO:0009228: thiamine biosynthetic process biological_process |
Author | Loftus BJ, Haft DH |
Entry Date | Apr 20 1999 2:05PM |
Last Modified | Jan 25 2012 12:12PM |
Comment | Members of this protein family are "ThiI", a sulfurtransferase involved in 4-thiouridine modification of tRNA. This protein often is bifunctional, with genetically separable activities, where the C-terminal rhodanese-like domain (residues 385 to 482 in E. coli ThiI), a domain not included in this model, is sufficient to synthesize the thiazole moiety of thiamine (see TIGR04271). Note that ThiI, because of its role in tRNA modification, may occur in species (such as Mycoplasma genitalium) that lack de novo thiamine biosynthesis. |
References | RN [1]
RM PMID:21724998
RT The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica.
RA Martinez-Gomez NC, Palmer LD, Vivas E, Roach PL, Downs DM
RL J Bacteriol. 2011 Sep;193(18):4582-7.
RN [2]
RM PMID:18604845
RT Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis.
RA You D, Xu T, Yao F, Zhou X, Deng Z
RL Chembiochem. 2008 Aug 11;9(12):1879-82.
RN [3]
RM 11443125
RT The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA.
RA Mueller EG, Palenchar PM, Buck CJ.
RL J Biol Chem. 2001 Sep 7;276(36):33588-95.
RN [4]
RM 97352701
RT Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.
RA Webb E, Claas K, Downs DM
RL J Bacteriol 1997 Jul;179(13):4399-402
DR PFAM; PF02568
DR InterPro; IPR003720
DR HAMAP; MF_00021; 184 of 191 |