| Accession | TIGR00049 |
| Name | TIGR00049 |
| Function | iron-sulfur cluster assembly accessory protein |
| Trusted Cutoff | 58.80 |
| Domain Trusted Cutoff | 58.80 |
| Noise Cutoff | 45.60 |
| Domain Noise Cutoff | 45.60 |
| Isology Type | subfamily |
| HMM Length | 106 |
| Mainrole Category | Biosynthesis of cofactors, prosthetic groups, and carriers |
| Subrole Category | Other |
| Gene Ontology Term | GO:0005198: structural molecule activity molecular_function |
| | GO:0016226: iron-sulfur cluster assembly biological_process |
| Author | Richardson DL, Haft DH |
| Entry Date | Apr 20 1999 2:04PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation [1]. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein [2]. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry [3]. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. |
| References | RN [1]
RM PMID: 12207230
RT The Rhizobium etli gene iscN is highly expressed in bacteroids and required for nitrogen fixation.
RA Dombrecht B, Tesfay MZ, Verreth C, Heusdens C, Napoles MC, Vanderleyden J, Michiels J.
RL Mol Genet Genomics 2002 Aug;267(6):820-8
RN [2]
RM PMID: 10322040
RT SufS is a NifS-like protein, and SufD is necessary for stability of the [2Fe-2S] FhuF protein in Escherichia coli.
RA Patzer SI, Hantke K.
RL J Bacteriol 1999 May;181(10):3307-9
RN [3]
RM PMID: 9582371
RT Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii.
RA Zheng L, Cash VL, Flint DH, Dean DR.
RL J Biol Chem 1998 May 22;273(21):13264-72
SE TIGR
DR HAMAP; MF_01429; 29 of 30 |
