Illumina (WGS) Assembly

Sequencing

As part of the HapMap project, whole genome shotgun sequence of M. truncatula (cultivar A17) was generated using Illumina technology (Illumina, San Diego).

The tables shown here represent the data released at the time of publication. Since then, more sequencing has taken place, and is available for BLAST search. Select "Illumina CLC assemblies (Feb/2011 unstable)" from the drop down menu.

Assembly

de novo assembly was generated using CLC-Bio assembler

Sequencing Run(s)	Read Length	Number of Reads
1x singleton	54bp	22,766,501 reads
3x short insert paired end (SIPE)	2x90bp	124,469,575 read pairs
1x long insert paired end (LIPE)	2x36bp	29,430,433 read pairs
Total: 26.98 Gb of sequence data (~54.0x genome coverage)

Total Number of contigs	143,160
Total length (bp)	250,625,650
Average size	1,750.67
Longest contig	30,515
N50	2,385
N80	1,104
Smallest contig	500

Filtering the contigs