| Accession | GenProp0953 |
| Name | O103(Ec) antigen biosynthesis |
| Type | METAPATH |
| Description | The E. coli O103 antigen structure has been determined [1], and the biosynthetic cluster analyzed [2]. The monomer unit is a linear pentasaccharide including one 3-deoxy-3-(R)-3-hydroxybutyramido-D-fucose per unit. The cluster includes four glycosyltransferases (WbtDEFG), but accurate assignment of which enzyme carries out each reaction has not yet been determined. The needed reactions are: 1) aDGalNAc-(1->3)-bDGlcNAc, 2) aDGlcNAc-(1->4)-aDGalNAc, 3) bDFuc3NBu-(1->6)-aDGlcNAc, and 4) bDGlc-(1->2)-bDFuc3NBu. |
| Parent Property | GenProp0913: E. coli O-antigen monomer biosynthesis clusters
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| Literature References | [ 1 ]Vinogradov E, Maclean LL, Perry MB Structural determination of the O-antigenic polysaccharide of enteropathogenic Escherichia coli O103:H2. Can J Microbiol. 2010 May;56(5):366-72. PMID 20555398
[ 2 ]Fratamico PM, DebRoy C, Strobaugh TP Jr, Chen CY DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes. Can J Microbiol. 2005 Jun;51(6):515-22. PMID 16121232
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| Step Name | Step Num | Required | Evidence (Method) | Evidence Go Terms |
|---|
| source of dTDP-DFuc3NBu | Fuc3NBu | YES | GenProp0952 (GENPROP): dTDP-3-deoxy-3-(R)-3-hydroxybutyramino-D-fucose biosynthesis from dTDP-3-aminofucose
| |
| O103(Ec)-associated glycosyltransferase WbtD | WbtD | YES | 2760 (RULE_BASE)
| |
| O103(Ec)-associated glycosyltransferase WbtE | WbtE | YES | 2761 (RULE_BASE)
| |
| O103(Ec)-associated glycosyltransferase WbtF | WbtF | YES | 2763 (RULE_BASE)
| |
| O103(Ec)-associated glycosyltransferase WbtG | WbtG | YES | 2764 (RULE_BASE)
| |
| O103(Ec)-specific transporter (flippase) wzx | wzx103 | YES | 2759 (RULE_BASE)
| |
| O103(Ec)-specific polymerase wzy | wzy103 | YES | 2762 (RULE_BASE)
| |
| chain length determinant protein | wzz | NO | PF02706 (HMM-CLUST): chain length determinant protein | |
