Genome Property Definition Page

Accession

GenProp0725

Name

outer membrane protein assembly complex

Type

METAPATH

Description

Correct assembly of proteins in the bacterial outer membrane is facilitated by a protein complex, consisting largely of lipoproteins, plus a member of the Omp85 protein family, named YaeT in Escherichia coli.

JCVI Role

Protein and peptide secretion and trafficking

Parent Property

GenProp0069: cell surface component
GenProp0248: protein folding

Literature References

[ 1 ]Malinverni JC, Werner J, Kim S, Sklar JG, Kahne D, Misra R, Silhavy TJ  YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli.  Mol Microbiol. 2006 Jul;61(1):151-64.  PMID 16824102
[ 2 ]Robert V, Volokhina EB, Senf F, Bos MP, Van Gelder P, Tommassen J  Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif.  PLoS Biol. 2006 Nov;4(11):e377.  PMID 17090219
[ 3 ] Charlson ES, Werner JN, Misra R   Differential effects of yfgL mutation on Escherichia coli outer membrane proteins and lipopolysaccharide.   J Bacteriol. 2006 Oct;188(20):7186-94.  PMID 17015657
[ 4 ]Hagan CL, Kim S, Kahne D  Reconstitution of outer membrane protein assembly from purified components.  Science. 2010 May 14;328(5980):890-2. Epub 2010 Apr 8.  PMID 20378773

Gene Ontology Term

GO:0006458: 'de novo' protein folding (biological_process)
GO:0006886: intracellular protein transport (biological_process)
GO:0032978: protein insertion into membrane from inner side (biological_process)
GO:0061077: chaperone-mediated protein folding (biological_process)

Components

Step Name	Step Num	Required	Evidence (Method)	Evidence Go Terms
OMP insertion complex protein BamA/YaeT	BamA	YES	TIGR03303 (HMM): outer membrane protein assembly complex, YaeT protein	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side
OMP insertion complex lipoprotein BamB/YfgL	BamB	NO	TIGR03300 (HMM): outer membrane assembly lipoprotein YfgL	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side
OMP insertion complex lipoprotein BamC/NlpB	BamC	NO	PF06804 (HMM): NlpB/DapX lipoprotein	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side
OMP insertion complex lipoprotein BamD/YfiO	BamD	YES	TIGR03302 (HMM): outer membrane assembly lipoprotein YfiO	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side
OMP insertion complex lipoprotein BamE/SmpA	BamE	NO	PF04355 (HMM): SmpA/OmlA family	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side
OMP insertion C-terminal signal	OM_targ	NO	TIGR03304 (HMM): outer membrane insertion C-terminal signal
OMP chaperone system: SurA-Skp-DegP	chaperon	YES	GenProp0928 (GENPROP): OMP chaperone system: SurA-Skp-DegP	GO:0006886: intracellular protein transport GO:0006458: 'de novo' protein folding GO:0061077: chaperone-mediated protein folding GO:0032978: protein insertion into membrane from inner side

Parent Properties

Accession	Name
GenProp0069	cell surface component
GenProp0248	protein folding

Child Properties

Accession	Name
GenProp0928	OMP chaperone system: SurA-Skp-DegP

Sibling Properties

Accession	Name
GenProp0007	flagella
GenProp0012	outer membrane
GenProp0022	S-layer
GenProp0056	type IV pilus
GenProp0062	capsule
GenProp0158	peptidoglycan(murein) biosynthesis
GenProp0297	lipopolysaccharide biosynthesis
GenProp0326	protein sorting system, PEP-CTERM/exosortase (generic)
GenProp0626	protein sorting system, sortase type, LPXTG/SrtA class
GenProp0664	protein sorting system, sortase type, SrtB class
GenProp0811	S-layer homology domain-mediated cell wall binding
GenProp0847	exosporium
GenProp0857	anchored repeat-class ABC transporter, P. acnes type
GenProp0859	protein sorting system, proteobacterial dedicated sortase type
GenProp0244	chaperone system: DnaK-DnaJ-GrpE
GenProp0245	chaperone system: GroEL/GroES
GenProp0246	chaperone system: thermosome/prefoldin
GenProp0928	OMP chaperone system: SurA-Skp-DegP